제품 > 항체 > 다중클론항체(pAb)

PLEK2 Rabbit pAb (A17682)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - PLEK2 Rabbit pAb (A17682)

Western blot analysis of various lysates using PLEK2 Rabbit pAb (A17682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePLEK2 Rabbit pAb
Catalog No.A17682
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. The encoded protein then interacts with the actin cytoskeleton to induce cell spreading. In conjunction with complement component 1, q subcomponent, B chain (C1QB), this gene shows an increase in expression in melanoma cells and may serve as an accurate biomarker for the disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PLEK2 (NP_057529.1).
SequenceMEDGVLKEGFLVKRGHIVHNWKARWFILRQNTLVYYKLEGGRRVTPPKGRILLDGCTITCPCLEYENRPLLIKLKTQTSTEYFLEACSREERDAWAFEITGAIHAGQPGKVQQLHSLRNSFKLPPHISLHRIVDKMHDSNTGIRSSPNMEQGSTYKKTFLGSSLVDWLISNSFTASRLEAVTLASMLMEENFLRPVGVRSMGAIRSGDLAEQFLDDSTALYTFAESYKKKISPKEEISLSTVELSGTVVK
Gene ID
Swiss Prot
SynonymsPLEK2
Calculated MW40kDa
Observed MW40kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse stomach, Mouse thymus
Cellular locationcytoplasm, cytoskeleton, lamellipodium membrane, plasma membrane
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PLEK2 Rabbit pAb (A17682)}

Western blot - PLEK2 Rabbit pAb (A17682)

Western blot analysis of various lysates using PLEK2 Rabbit pAb (A17682) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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