제품 > 항체 > 다중클론항체(pAb)

PLEKHB2 Rabbit pAb (A15462)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - PLEKHB2 Rabbit pAb (A15462)

Western blot analysis of various lysates using PLEKHB2 Rabbit pAb (A15462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PLEKHB2 Rabbit pAb (A15462)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PLEKHB2 Rabbit pAb (A15462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePLEKHB2 Rabbit pAb
Catalog No.A15462
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables phosphatidylinositol-3, 4, 5-trisphosphate binding activity. Predicted to be involved in regulation of cell differentiation. Predicted to be located in recycling endosome membrane. Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human PLEKHB2 (NP_001094093.1).
SequenceMAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECRDTQPPDGKSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQDSRTNTAYVGSAVMTDETSVVSSPPPYTAYAAPAPEQAYGYGPYGGAYPPGTQVVYAANGQAYAVPYQYPYAGLYGQQPANQVIIRERYRDNDSDLALGMLAGAATGMALGSLFWVF
Gene ID
Swiss Prot
SynonymsEVT2; PLEKHB2
Calculated MW25kDa
Observed MW15kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHT-29, A-549, BxPC-3, A-431
Cellular locationPeripheral membrane protein, Recycling endosome membrane

Documents

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    ABclonal:Western blot - PLEKHB2 Rabbit pAb (A15462)}

    Western blot - PLEKHB2 Rabbit pAb (A15462)

    Western blot analysis of various lysates using PLEKHB2 Rabbit pAb (A15462) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - PLEKHB2 Rabbit pAb (A15462)}

    Immunohistochemistry - PLEKHB2 Rabbit pAb (A15462)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PLEKHB2 Rabbit pAb (A15462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.