제품 > 항체 > 다중클론항체(pAb)

PLLP Rabbit pAb (A15834)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PLLP Rabbit pAb (A15834)

Western blot analysis of various lysates using PLLP Rabbit pAb (A15834) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - PLLP Rabbit pAb (A15834)

Immunofluorescence analysis of L929 cells using PLLP Rabbit pAb (A15834) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePLLP Rabbit pAb
Catalog No.A15834
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be a structural constituent of myelin sheath. Predicted to be involved in myelination. Predicted to be located in compact myelin and membrane raft. Predicted to be integral component of membrane. Biomarker of schizophrenia.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PLLP (NP_057077.1).
SequenceMAEFPSKVSTRTSSPAQGAEASVSALRPDLGFVRSRLGALMLLQLVLGLLVWALIADTPYHLYPAYGWVMFVAVFLWLVTIVLFNLYLFQLHMKLYMVPW
Gene ID
Swiss Prot
SynonymsPMLP; TM4SF11; PLLP
Calculated MW20kDa
Observed MW20kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain, Rat kidney
Cellular locationMembrane, Multi-pass membrane protein

Documents

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    ABclonal:Western blot - PLLP Rabbit pAb (A15834)}

    Western blot - PLLP Rabbit pAb (A15834)

    Western blot analysis of various lysates using PLLP Rabbit pAb (A15834) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - PLLP Rabbit pAb (A15834)}

    Immunofluorescence - PLLP Rabbit pAb (A15834)

    Immunofluorescence analysis of L929 cells using PLLP Rabbit pAb (A15834) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.