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PML Rabbit pAb (A18182)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PML Rabbit pAb (A18182)

Western blot analysis of various lysates using PML Rabbit pAb (A18182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - PML Rabbit pAb (A18182)

Immunohistochemistry analysis of paraffin-embedded human appendix using PML Rabbit pAb (A18182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePML Rabbit pAb
Catalog No.A18182
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 571-611 of human PML (NP_150241.2).
SequenceSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLV
Gene ID
Swiss Prot
SynonymsMYL; RNF71; PP8675; TRIM19; PML
Calculated MW98kDa
Observed MW67kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, Mouse spleen, Mouse thymus, Rat kidney
Cellular locationCytoplasm, Cytoplasmic side, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, PML body, Peripheral membrane protein, nucleolus, nucleoplasm

Documents

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ABclonal:Western blot - PML Rabbit pAb (A18182)}

Western blot - PML Rabbit pAb (A18182)

Western blot analysis of various lysates using PML Rabbit pAb (A18182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - PML Rabbit pAb (A18182)}

Immunohistochemistry - PML Rabbit pAb (A18182)

Immunohistochemistry analysis of paraffin-embedded human appendix using PML Rabbit pAb (A18182) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)