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PNKP Rabbit pAb (A6693)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PNKP Rabbit pAb (A6693)

Western blot analysis of various lysates using PNKP Rabbit pAb (A6693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PNKP Rabbit pAb (A6693)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP Rabbit pAb (A6693). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Overview

Product namePNKP Rabbit pAb
Catalog No.A6693
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
SequenceRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLP
Gene ID
Swiss Prot
SynonymsPNK; AOA4; MCSZ; CMT2B2; EIEE10; PNKP
Calculated MW57kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60, HepG2, Rat liver
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PNKP Rabbit pAb (A6693)}

Western blot - PNKP Rabbit pAb (A6693)

Western blot analysis of various lysates using PNKP Rabbit pAb (A6693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)}

Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)}

Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PNKP Rabbit pAb (A6693)}

Immunohistochemistry - PNKP Rabbit pAb (A6693)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using PNKP Rabbit pAb (A6693) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PNKP Rabbit pAb (A6693)}

Immunofluorescence - PNKP Rabbit pAb (A6693)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP Rabbit pAb (A6693). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6693? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)