제품 > 항체 > 다중클론항체(pAb)

PNLIPRP2 Rabbit pAb (A12895)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PNLIPRP2 Rabbit pAb (A12895)

Western blot analysis of lysates from rat pancreas, using PNLIPRP2 Rabbit pAb (A12895) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)

Immunofluorescence analysis of paraffin-embedded rat pancreas using PNLIPRP2 Rabbit pAb (A12895) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using PNLIPRP2 Rabbit pAb (A12895) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePNLIPRP2 Rabbit pAb
Catalog No.A12895
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 301-470 of human PNLIPRP2 (NP_005387.2).
SequenceLGYPCASYDEFQESKCFPCPAEGCPKMGHYADQFKGKTSAVEQTFFLNTGESGNFTSWRYKVSVTLSGKEKVNGYIRIALYGSNENSKQYEIFKGSLKPDASHTCAIDVDFNVGKIQKVKFLWNKRGINLSEPKLGASQITVQSGEDGTEYNFCSSDTVEENVLQSLYPC
Gene ID
Swiss Prot
SynonymsPLRP2; PNLIPRP2
Calculated MW52kDa
Observed MW54kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat pancreas
Cellular locationSecreted

Documents

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Lot number

    ABclonal:Western blot - PNLIPRP2 Rabbit pAb (A12895)}

    Western blot - PNLIPRP2 Rabbit pAb (A12895)

    Western blot analysis of lysates from rat pancreas, using PNLIPRP2 Rabbit pAb (A12895) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)}

    Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)

    Immunofluorescence analysis of paraffin-embedded rat pancreas using PNLIPRP2 Rabbit pAb (A12895) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)}

    Immunofluorescence - PNLIPRP2 Rabbit pAb (A12895)

    Immunofluorescence analysis of paraffin-embedded mouse pancreas using PNLIPRP2 Rabbit pAb (A12895) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.