제품 > 항체 > 다중클론항체(pAb)

POFUT2 Rabbit pAb (A12223)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - POFUT2 Rabbit pAb (A12223)

Western blot analysis of various lysates using POFUT2 Rabbit pAb (A12223) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - POFUT2 Rabbit pAb (A12223)

Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (A12223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - POFUT2 Rabbit pAb (A12223)

Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (A12223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePOFUT2 Rabbit pAb
Catalog No.A12223
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).
SequenceMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAII
Gene ID
Swiss Prot
SynonymsFUT13; C21orf80; POFUT2
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, HepG2, Mouse brain, Mouse heart, Rat brain, Rat heart
Cellular locationEndoplasmic reticulum, Golgi apparatus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - POFUT2 Rabbit pAb (A12223)}

    Western blot - POFUT2 Rabbit pAb (A12223)

    Western blot analysis of various lysates using POFUT2 Rabbit pAb (A12223) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - POFUT2 Rabbit pAb (A12223)}

    Immunofluorescence - POFUT2 Rabbit pAb (A12223)

    Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (A12223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - POFUT2 Rabbit pAb (A12223)}

    Immunofluorescence - POFUT2 Rabbit pAb (A12223)

    Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (A12223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.