제품 > 항체 > 다중클론항체(pAb)

POLD1 Rabbit pAb (A5323)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using POLD1 Rabbit pAb (A5323) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - POLD1 Rabbit pAb (A5323)

Immunofluorescence analysis of U2OS cells using POLD1 Rabbit pAb (A5323). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using POLD1 Rabbit pAb (A5323).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Immunofluorescence analysis of U2OS cells using POLD1 Rabbit pAb (A5323). Green：gamma H2AX (phospho S139) used for DNA damage marker. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Overview

Product name	POLD1 Rabbit pAb
Catalog No.	A5323
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).
Sequence	RYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW
Gene ID
Swiss Prot
Synonyms	CDC2; MDPL; POLD; CRCS10; POLD1
Calculated MW	124kDa
Observed MW	130kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, SW480, Mouse spleen
Cellular location	Nucleus
Customer validation	WB(Homo sapiens) IF(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

POLD1 Rabbit pAb images

Western blot - POLD1 Rabbit pAb (A5323)

Immunofluorescence - POLD1 Rabbit pAb (A5323)

Immunofluorescence analysis of U2OS cells using POLD1 Rabbit pAb (A5323). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence - POLD1 Rabbit pAb (A5323)

Immunofluorescence analysis of A549 cells using POLD1 Rabbit pAb (A5323).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Immunofluorescence - POLD1 Rabbit pAb (A5323)

* For research use only. Not for therapeutic or diagnostic purposes.