제품 > 항체 > 다중클론항체(pAb)

POLR1E Rabbit pAb (A12700)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR1E Rabbit pAb (A12700)

Western blot analysis of various lysates using POLR1E Rabbit pAb (A12700) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - POLR1E Rabbit pAb (A12700)

Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg POLR1E antibody (A12700). Western blot was performed from the immunoprecipitate using POLR1E antibody (A12700) at a dilution of 1:1000.

Overview

Product namePOLR1E Rabbit pAb
Catalog No.A12700
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA binding activity; DNA-directed 5'-3' RNA polymerase activity; and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I. Located in fibrillar center and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
SequenceMAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIEAFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDFQIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT
Gene ID
Swiss Prot
SynonymsA49; PAF53; PRAF1; RPA49; POLR1E
Calculated MW47kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa, A-549, 293T, Jurkat
Cellular locationNucleus, nucleolus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - POLR1E Rabbit pAb (A12700)}

    Western blot - POLR1E Rabbit pAb (A12700)

    Western blot analysis of various lysates using POLR1E Rabbit pAb (A12700) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunoprecipitation - POLR1E Rabbit pAb (A12700)}

    Immunoprecipitation - POLR1E Rabbit pAb (A12700)

    Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg POLR1E antibody (A12700). Western blot was performed from the immunoprecipitate using POLR1E antibody (A12700) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12700? Please let us know so that we can cite the reference in this datasheet.