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PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Western blot analysis of various lysates using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded rat kidney using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded human esophagus using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded mouse liver using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePP2A-B55α/PR55α/PPP2R2A Rabbit pAb
Catalog No.A2185
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PP2A-B55α/PR55α/PPP2R2A (NP_002708.1).
SequenceMAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN
Gene ID
Swiss Prot
SynonymsB55A; PR52A; PR55A; B55ALPHA; PR55alpha; PP2A-B55α/PR55α/PPP2R2A
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, Mouse brain
Cellular locationcytosol, glutamatergic synapse, nucleoplasm
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)}

Western blot - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Western blot analysis of various lysates using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)}

Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded rat kidney using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)}

Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded human esophagus using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)}

Immunohistochemistry - PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185)

Immunohistochemistry analysis of paraffin-embedded mouse liver using PP2A-B55α/PR55α/PPP2R2A Rabbit pAb (A2185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2185? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)