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PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb (A19333)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb (A19333)

Western blot analysis of lysates from Rat spleen using PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb(A19333) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product namePP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb
Catalog No.A19333
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human PP2A-B56δ/PR61δ/PPP2R5E (NP_006237.1).
SequenceLLKDVPSSEQPELFLKKLQQCCVIFDFMDTLSDLKMKEYKRSTLNELVDYITISRGCLTEQTYPEVVRMVSCNIFRTLPPSDSNEFDPEEDEPTLEASWPH
Gene ID
Swiss Prot
SynonymsB56E; B56epsilon; PP2A-B56δ/PR61δ/PPP2R5E
Calculated MW55kDa
Observed MW49kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat spleen
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb (A19333)}

Western blot - PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb (A19333)

Western blot analysis of lysates from Rat spleen using PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb(A19333) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)