제품 > 항체 > 다중클론항체(pAb)

PPIL1 Rabbit pAb (A14892)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PPIL1 Rabbit pAb (A14892)

Western blot analysis of various lysates using PPIL1 Rabbit pAb (A14892) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePPIL1 Rabbit pAb
Catalog No.A14892
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human PPIL1 (NP_057143.1).
SequenceMAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAMANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQDRPVDDVKIIKAYPSG
Gene ID
Swiss Prot
SynonymsCYPL1; PCH14; hCyPX; PPIase; CGI-124; PPIL1
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, HeLa, Raji, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat lung, Rat spleen
Cellular locationnucleoplasm, nucleus

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    ABclonal:Western blot - PPIL1 Rabbit pAb (A14892)}

    Western blot - PPIL1 Rabbit pAb (A14892)

    Western blot analysis of various lysates using PPIL1 Rabbit pAb (A14892) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.