제품 > 항체 > 다중클론항체(pAb)

PPP1R14B Rabbit pAb (A14677)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP1R14B Rabbit pAb (A14677)

Western blot analysis of various lysates using PPP1R14B Rabbit pAb (A14677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product namePPP1R14B Rabbit pAb
Catalog No.A14677
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein serine/threonine phosphatase inhibitor activity. Predicted to be involved in innate immune response. Predicted to be located in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 68-147 of human PPP1R14B (NP_619634.1).
SequenceRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK
Gene ID
Swiss Prot
SynonymsPNG; PHI-1; PLCB3N; SOM172; PPP1R14B
Calculated MW16kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A-431
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - PPP1R14B Rabbit pAb (A14677)}

    Western blot - PPP1R14B Rabbit pAb (A14677)

    Western blot analysis of various lysates using PPP1R14B Rabbit pAb (A14677) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A14677? Please let us know so that we can cite the reference in this datasheet.