제품 > 항체 > 다중클론항체(pAb)

PPP1R9B Rabbit pAb (A17230)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using PPP1R9B Rabbit pAb (A17230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - PPP1R9B Rabbit pAb (A17230)

Immunofluorescence analysis of L929 cells using PPP1R9B Rabbit pAb (A17230) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	PPP1R9B Rabbit pAb
Catalog No.	A17230
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PPP1R9B (NP_115984.3).
Sequence	VFQPPPPPPPAPSGDAPAEKERCPAGQQPPQHRVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGSTVATAASPAPEEPKAQA
Gene ID
Swiss Prot
Synonyms	Spn; SPINO; PPP1R6; PPP1R9; PPP1R9B
Calculated MW	89kDa
Observed MW	127kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87MG, K-562, Mouse brain
Cellular location	Cell junction, Cell membrane, Cell projection, Cytoplasm, Nucleus, adherens junction, cytoskeleton, dendritic spine, filopodium, lamellipodium, ruffle membrane, synapse