제품 > 항체 > 다중클론항체(pAb)

PPP3R1 Rabbit pAb (A0954)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP3R1 Rabbit pAb (A0954)

Western blot analysis of extracts of various cell lines, using PPP3R1 antibody (A0954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - PPP3R1 Rabbit pAb (A0954)

Immunofluorescence analysis of NIH/3T3 cells using PPP3R1 antibody (A0954) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePPP3R1 Rabbit pAb
Catalog No.A0954
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimer's disease and dilated cardiomyopathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_000936.1).
SequenceMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV
Gene ID
Swiss Prot
SynonymsCNB; CNB1; CALNB1
Calculated MW19kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Mouse spleen, Mouse lung, Rat brain, SH-SY5Y, SW480, HL-60, MCF7, Rat testis
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Membrane, cytosol, sarcolemma

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ABclonal:Western blot - PPP3R1 Rabbit pAb (A0954)}

Western blot - PPP3R1 Rabbit pAb (A0954)

Western blot analysis of extracts of various cell lines, using PPP3R1 antibody (A0954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - PPP3R1 Rabbit pAb (A0954)}

Immunofluorescence - PPP3R1 Rabbit pAb (A0954)

Immunofluorescence analysis of NIH/3T3 cells using PPP3R1 antibody (A0954) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.