제품 > 항체 > 다중클론항체(pAb)

PPP4R3B Rabbit pAb (A17189)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP4R3B Rabbit pAb (A17189)

Western blot analysis of lysates from Mouse testis, using PPP4R3B Rabbit pAb (A17189) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - PPP4R3B Rabbit pAb (A17189)

Immunofluorescence analysis of U-2 OS cells using PPP4R3B Rabbit pAb (A17189) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePPP4R3B Rabbit pAb
Catalog No.A17189
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to act upstream of or within positive regulation of gluconeogenesis and protein dephosphorylation. Located in centrosome and nuclear speck.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 628-817 of human PPP4R3B (NP_001269779.1).
SequenceYKALESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVVAPVEKPKPEDDFPDNYEKFMETKKAKESEDKENLPKRTSPGGFKFTFSHSASAANGTNSKSVVAQIPPATSNGSSSKTTNLPTSVTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS
Gene ID
Swiss Prot
SynonymsPSY2; smk1; FLFL2; SMEK2; PP4R3B; PPP4R3B
Calculated MW97kDa
Observed MW97kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Documents

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    ABclonal:Western blot - PPP4R3B Rabbit pAb (A17189)}

    Western blot - PPP4R3B Rabbit pAb (A17189)

    Western blot analysis of lysates from Mouse testis, using PPP4R3B Rabbit pAb (A17189) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - PPP4R3B Rabbit pAb (A17189)}

    Immunofluorescence - PPP4R3B Rabbit pAb (A17189)

    Immunofluorescence analysis of U-2 OS cells using PPP4R3B Rabbit pAb (A17189) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)