제품 > 항체 > 다중클론항체(pAb)

PRDM1 Rabbit pAb (A1960)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PRDM1 Rabbit pAb (A1960)

Western blot analysis of extracts of various cell lines, using (A1960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - PRDM1 Rabbit pAb (A1960)

Immunofluorescence analysis of A-431 cells using PRDM1 Rabbit pAb (A1960) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePRDM1 Rabbit pAb
Catalog No.A1960
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 70-210 of human PRDM1 (NP_001189.2).
SequenceGADGGTSVQAEASLPRNLLFKYATNSEEVIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWRIYSRGELHHFIDGFNEEKSNWMRYVNPAHSPREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHY
Gene ID
Swiss Prot
SynonymsBLIMP1; PRDI-BF1
Calculated MW92kDa
Observed MW100kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, Mouse liver
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - PRDM1 Rabbit pAb (A1960)}

Western blot - PRDM1 Rabbit pAb (A1960)

Western blot analysis of extracts of various cell lines, using (A1960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - PRDM1 Rabbit pAb (A1960)}

Immunofluorescence - PRDM1 Rabbit pAb (A1960)

Immunofluorescence analysis of A-431 cells using PRDM1 Rabbit pAb (A1960) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.