제품 > 항체 > 단일클론항체(mAb)

PREB Rabbit mAb (A20957)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PREB Rabbit mAb (A20957)

Western blot analysis of extracts of various cell lines, using PREB antibody (A20957) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PREB Rabbit mAb (A20957)

Western blot analysis of extracts of various cell lines, using PREB antibody (A20957) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - PREB Rabbit mAb (A20957)

Immunofluorescence analysis of NIH/3T3 cells using PREB Rabbit mAb (A20957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PREB Rabbit mAb (A20957)

Immunofluorescence analysis of PC-12 cells using PREB Rabbit mAb (A20957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePREB Rabbit mAb
Catalog No.A20957
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2945
This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 89-201 of human PREB (NP_037520.1).
SequenceHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELRVENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIED
Gene ID
Swiss Prot
SynonymsSEC12
Calculated MW45kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHep G2, MCF7, K-562, Mouse brain, Rat brain
Cellular locationEndoplasmic reticulum membrane, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PREB Rabbit mAb (A20957)}

    Western blot - PREB Rabbit mAb (A20957)

    Western blot analysis of extracts of various cell lines, using PREB antibody (A20957) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - PREB Rabbit mAb (A20957)}

    Western blot - PREB Rabbit mAb (A20957)

    Western blot analysis of extracts of various cell lines, using PREB antibody (A20957) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - PREB Rabbit mAb (A20957)}

    Immunofluorescence - PREB Rabbit mAb (A20957)

    Immunofluorescence analysis of NIH/3T3 cells using PREB Rabbit mAb (A20957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PREB Rabbit mAb (A20957)}

    Immunofluorescence - PREB Rabbit mAb (A20957)

    Immunofluorescence analysis of PC-12 cells using PREB Rabbit mAb (A20957) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)