제품 > 항체 > 다중클론항체(pAb)

PRKG2 Rabbit pAb (A18575)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PRKG2 Rabbit pAb (A18575)

Western blot analysis of various lysates using PRKG2 Rabbit pAb (A18575) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - PRKG2 Rabbit pAb (A18575)

Immunoprecipitation analysis of 600 μg extracts of Mouse lung using 3 μg PRKG2 antibody (A18575). Western blot was performed from the immunoprecipitate using PRKG2 antibody (A18575) at a dilution of 1:1000.

Overview

Product namePRKG2 Rabbit pAb
Catalog No.A18575
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PRKG2 (NP_006250.1).
SequenceMGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLIT
Gene ID
Swiss Prot
SynonymsAMD4; PKG2; SMDP; cGK2; cGKII; PRKGR2; PRKG2
Calculated MW87kDa
Observed MW100kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse lung, Rat brain
Cellular locationApical cell membrane, Lipid-anchor

Documents

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    ABclonal:Western blot - PRKG2 Rabbit pAb (A18575)}

    Western blot - PRKG2 Rabbit pAb (A18575)

    Western blot analysis of various lysates using PRKG2 Rabbit pAb (A18575) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunoprecipitation - PRKG2 Rabbit pAb (A18575)}

    Immunoprecipitation - PRKG2 Rabbit pAb (A18575)

    Immunoprecipitation analysis of 600 μg extracts of Mouse lung using 3 μg PRKG2 antibody (A18575). Western blot was performed from the immunoprecipitate using PRKG2 antibody (A18575) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.