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PRPF4 Rabbit mAb (A21132)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PRPF4 Rabbit mAb (A21132)

Western blot analysis of various lysates using PRPF4 Rabbit mAb (A21132) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PRPF4 Rabbit mAb (A21132)

Western blot analysis of lysates from Rat liver, using PRPF4 Rabbit mAb (A21132) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

Immunohistochemistry analysis of paraffin-embedded human esophagus using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

Immunohistochemistry analysis of paraffin-embedded rat ovary using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product namePRPF4 Rabbit mAb
Catalog No.A21132
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3034
The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 450-522 of human PRPF4 (NP_004688.2).
SequenceNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE
Gene ID
Swiss Prot
SynonymsPRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60; PRPF4
Calculated MW58kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Mouse spleen, Mouse thymus, Rat liver
Cellular locationNucleus speckle

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - PRPF4 Rabbit mAb (A21132)}

    Western blot - PRPF4 Rabbit mAb (A21132)

    Western blot analysis of various lysates using PRPF4 Rabbit mAb (A21132) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - PRPF4 Rabbit mAb (A21132)}

    Western blot - PRPF4 Rabbit mAb (A21132)

    Western blot analysis of lysates from Rat liver, using PRPF4 Rabbit mAb (A21132) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)}

    Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)}

    Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

    Immunohistochemistry analysis of paraffin-embedded human esophagus using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)}

    Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

    Immunohistochemistry analysis of paraffin-embedded mouse spleen using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPF4 Rabbit mAb (A21132)}

    Immunohistochemistry - PRPF4 Rabbit mAb (A21132)

    Immunohistochemistry analysis of paraffin-embedded rat ovary using PRPF4 Rabbit mAb (A21132) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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