제품 > 항체 > 다중클론항체(pAb)

PRPSAP1 Rabbit pAb (A15710)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PRPSAP1 Rabbit pAb (A15710)

Western blot analysis of various lysates using PRPSAP1 Rabbit pAb (A15710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePRPSAP1 Rabbit pAb
Catalog No.A15710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Predicted to be involved in 5-phosphoribose 1-diphosphate biosynthetic process and purine nucleotide biosynthetic process. Predicted to be part of ribose phosphate diphosphokinase complex. Predicted to be active in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-110 of human PRPSAP1 (NP_002757.2).
SequenceMNAARTGYRVFSANSTAACTELAKRITERLGAELGKSVVYQETNGETRVEIKESVRGQDIFIIQTIPRDVNTAVMELLIMA
Gene ID
Swiss Prot
SynonymsPAP39; PRPSAP1
Calculated MW39kDa
Observed MW42kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse heart
Cellular locationcytoplasm
    ABclonal:Western blot - PRPSAP1 Rabbit pAb (A15710)}

    Western blot - PRPSAP1 Rabbit pAb (A15710)

    Western blot analysis of various lysates using PRPSAP1 Rabbit pAb (A15710) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.