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PRPSAP2 Rabbit pAb (A15711)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PRPSAP2 Rabbit pAb (A15711)

Western blot analysis of various lysates using PRPSAP2 Rabbit pAb (A15711) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

Immunohistochemistry analysis of paraffin-embedded mouse brain using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

Immunohistochemistry analysis of paraffin-embedded rat ovary using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

Immunohistochemistry analysis of paraffin-embedded rat spleen using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product namePRPSAP2 Rabbit pAb
Catalog No.A15711
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that associates with the enzyme phosphoribosylpyrophosphate synthetase (PRS). PRS catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 211-252 of human PRPSAP2 (NP_001340030.1).
SequenceHGEAQDAESDLVDGRHSPPMVRSVAAIHPSLEIPMLIPKEKP
Gene ID
Swiss Prot
SynonymsPAP41; PRPSAP2
Calculated MW41kDa
Observed MW36kDa/41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular locationcytoplasm

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PRPSAP2 Rabbit pAb (A15711)}

    Western blot - PRPSAP2 Rabbit pAb (A15711)

    Western blot analysis of various lysates using PRPSAP2 Rabbit pAb (A15711) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)}

    Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

    Immunohistochemistry analysis of paraffin-embedded mouse brain using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)}

    Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

    Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)}

    Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

    Immunohistochemistry analysis of paraffin-embedded rat ovary using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)}

    Immunohistochemistry - PRPSAP2 Rabbit pAb (A15711)

    Immunohistochemistry analysis of paraffin-embedded rat spleen using PRPSAP2 Rabbit pAb (A15711) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.