제품 > 항체 > 다중클론항체(pAb)

PRR5 Rabbit pAb (A22252)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of lysates from Inflorescences from Arabidopsis thaliana, using PRR5 Rabbit pAb (A22252) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	PRR5 Rabbit pAb
Catalog No.	A22252
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Encodes a pseudo-response regulator whose mutation affects various circadian-associated biological events such as flowering time in the long-day photoperiod conditions, red light sensitivity of seedlings during early photomorphogenesis, and the period of free-running rhythms of certain clock-controlled genes including CCA1 and APRR1/TOC1 in constant white light. Acts as transcriptional repressor of CCA1 and LHY.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PRR5 (NP_568446.2).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	APRR5; pseudo-response regulator 5; T31K7.5; T31K7_5; PRR5
Calculated MW	62kDa
Observed MW	62kDa

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Inflorescences
Cellular location	nucleus