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PSMA1 Rabbit pAb (A2523)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSMA1 Rabbit pAb (A2523)

Western blot analysis of extracts of various cell lines, using PSMA1 antibody (A2523) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - PSMA1 Rabbit pAb (A2523)

Immunofluorescence analysis of U2OS cells using PSMA1 antibody (A2523) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePSMA1 Rabbit pAb
Catalog No.A2523
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMA1 (NP_002777.1).
SequenceMFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH
Gene ID
Swiss Prot
SynonymsNU; HC2; PROS30; HEL-S-275
Calculated MW30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, Jurkat, HeLa, HepG2, U-251MG, SW620, Mouse liver, Mouse heart, Mouse spleen
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PSMA1 Rabbit pAb (A2523)}

    Western blot - PSMA1 Rabbit pAb (A2523)

    Western blot analysis of extracts of various cell lines, using PSMA1 antibody (A2523) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Immunofluorescence - PSMA1 Rabbit pAb (A2523)}

    Immunofluorescence - PSMA1 Rabbit pAb (A2523)

    Immunofluorescence analysis of U2OS cells using PSMA1 antibody (A2523) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)