제품 > 항체 > 다중클론항체(pAb)

PSMA4 Rabbit pAb (A2511)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PSMA4 Rabbit pAb (A2511) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - PSMA4 Rabbit pAb (A2511)

Immunofluorescence analysis of U2OS cells using PSMA4 Rabbit pAb (A2511). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	PSMA4 Rabbit pAb
Catalog No.	A2511
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a core alpha subunit of the 20S proteosome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. Proteasomes cleave peptides in an ATP- and ubiquitin-dependent manner.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PSMA4 (NP_001096137.1).
Sequence	MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK
Gene ID
Swiss Prot
Synonyms	HC9; PSC9; HsT17706; PSMA4
Calculated MW	29kDa
Observed MW	28kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, U-251MG, HepG2, HeLa, Jurkat, PC-12, Mouse liver, Mouse kidney, COS-7
Cellular location	Cytoplasm, Nucleus, P-body
Customer validation	(293T、HeLa、Jurkat、T47D、U251、SKVO-3、MOLT-4、HepG2、PC-3, PC-3 HEPG2 HELA MOLT4 JURKAT U251 T47D 293T)

Documents

Certificate of Compliance

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Research Area

PSMA4 Rabbit pAb images

Western blot - PSMA4 Rabbit pAb (A2511)

Immunofluorescence - PSMA4 Rabbit pAb (A2511)

Immunofluorescence analysis of U2OS cells using PSMA4 Rabbit pAb (A2511). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.