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PSMA5 Rabbit pAb (A14053)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSMA5 Rabbit pAb (A14053)

Western blot analysis of various lysates using PSMA5 Rabbit pAb (A14053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunoprecipitation - PSMA5 Rabbit pAb (A14053)

Immunoprecipitation analysis of 600 μg extracts of Mouse liver cells using 3 μg PSMA5 antibody (A14053). Western blot was performed from the immunoprecipitate using PSMA5 antibody (A14053) at a dilution of 1:1000.

Overview

Product namePSMA5 Rabbit pAb
Catalog No.A14053
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human PSMA5 (NP_002781.2).
SequenceMFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI
Gene ID
Swiss Prot
SynonymsPSC5; ZETA; PSMA5
Calculated MW26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesMCF7, 293T, HepG2, U-251MG, BxPC-3, Mouse liver, Mouse thymus, Mouse heart
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PSMA5 Rabbit pAb (A14053)}

    Western blot - PSMA5 Rabbit pAb (A14053)

    Western blot analysis of various lysates using PSMA5 Rabbit pAb (A14053) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Immunoprecipitation - PSMA5 Rabbit pAb (A14053)}

    Immunoprecipitation - PSMA5 Rabbit pAb (A14053)

    Immunoprecipitation analysis of 600 μg extracts of Mouse liver cells using 3 μg PSMA5 antibody (A14053). Western blot was performed from the immunoprecipitate using PSMA5 antibody (A14053) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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