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PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Western blot analysis of various lysates using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Overview

Product namePSMA/FOLH1 Rabbit PolymAb®
Catalog No.A26428PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human PSMA/FOLH1 (NP_004467.1).
SequenceTNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPG
Gene ID
Swiss Prot
SynonymsPSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1
Calculated MW84kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:6000
  • IHC-P 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat brain
Cellular locationCell membrane, Cytoplasm, Single-pass type II membrane protein, cell surface, cytoplasm, extracellular exosome, plasma membrane.

Documents

Certificate of Compliance

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ABclonal:Western blot - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)}

Western blot - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Western blot analysis of various lysates using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)}

Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)}

Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)}

Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)}

Immunohistochemistry - PSMA/FOLH1 Rabbit PolymAb® (A26428PM)

Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using PSMA/FOLH1 Rabbit PolymAb® (A26428PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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