제품 > 항체 > 다중클론항체(pAb)

PSMB3 Rabbit pAb (A9947)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSMB3 Rabbit pAb (A9947)

Western blot analysis of various lysates using PSMB3 Rabbit pAb (A9947) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

Immunohistochemistry analysis of paraffin-embedded Rat lung using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePSMB3 Rabbit pAb
Catalog No.A9947
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PSMB3 (NP_002786.2).
SequenceMSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD
Gene ID
Swiss Prot
SynonymsHC10-II; PSMB3
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3, MCF7, HeLa, Jurkat, Mouse brain, Rat liver, Rat brain
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PSMB3 Rabbit pAb (A9947)}

    Western blot - PSMB3 Rabbit pAb (A9947)

    Western blot analysis of various lysates using PSMB3 Rabbit pAb (A9947) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)}

    Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)}

    Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PSMB3 Rabbit pAb (A9947)}

    Immunohistochemistry - PSMB3 Rabbit pAb (A9947)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PSMB3 Rabbit pAb (A9947) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)