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PSMB9/LMP2 Rabbit pAb (A1771)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - PSMB9/LMP2 Rabbit pAb (A1771)

Western blot analysis of various lysates using PSMB9/LMP2 Rabbit pAb (A1771) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.

ABclonal:Immunofluorescence - PSMB9/LMP2 Rabbit pAb (A1771)

Immunofluorescence analysis of MCF-7 cells using PSMB9/LMP2 Rabbit pAb (A1771).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product namePSMB9/LMP2 Rabbit pAb
Catalog No.A1771
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human PSMB9/LMP2 (NP_002791.1).
SequenceMLRAGAPTGDLPRAGEVHTGTTIMAVEFDGGVVMGSDSRVSAGEAVVNRVFDKLSPLHERIYCALSGSAADAQAVADMAAYQLELHGIELEEPPLVLAAANVVRNISYKYREDLSAHLMVAGWDQREGGQVYGTLGGMLTRQPFAIGGSGSTFIYGYVDAAYKPGMSPEECRRFTTDAIALAMSRDGSSGGVIYLVTITAAGVDHRVILGNELPKFYDE
Gene ID
Swiss Prot
SynonymsLMP2; PRAAS3; PSMB6i; RING12; beta1i; PSMB9/LMP2
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRaji, THP-1
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PSMB9/LMP2 Rabbit pAb (A1771)}

    Western blot - PSMB9/LMP2 Rabbit pAb (A1771)

    Western blot analysis of various lysates using PSMB9/LMP2 Rabbit pAb (A1771) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime: 90s.
    ABclonal:Immunofluorescence - PSMB9/LMP2 Rabbit pAb (A1771)}

    Immunofluorescence - PSMB9/LMP2 Rabbit pAb (A1771)

    Immunofluorescence analysis of MCF-7 cells using PSMB9/LMP2 Rabbit pAb (A1771).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1771? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    ELISA 키트 (1)

    Secondary Antibodies (25)