제품 > 항체 > 다중클론항체(pAb)

PSTPIP1 Rabbit pAb (A7760)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSTPIP1 Rabbit pAb (A7760)

Western blot analysis of various lysates using PSTPIP1 Rabbit pAb (A7760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

ABclonal:Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)

Immunohistochemistry analysis of paraffin-embedded Human stomach using PSTPIP1 Rabbit pAb (A7760) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PSTPIP1 Rabbit pAb (A7760) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePSTPIP1 Rabbit pAb
Catalog No.A7760
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 157-416 of human PSTPIP1 (NP_003969.2).
SequenceAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIAVQEIQGNPASPAQEYRALYDYTAQNPDELDLSAGDILEVILEGEDGWWTVERNGQRGFVPGSYLEKL
Gene ID
Swiss Prot
SynonymsH-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S; PSTPIP1
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesTHP-1, mouse spleen, mouse thymus, mouse brain, rat brain, Mouse spleen, Mouse thymus, Mouse brain, Rat brain
Cellular locationCell projection, Cleavage furrow, Cytoplasm, cytoskeleton, lamellipodium, perinuclear region, uropodium

Documents

Certificate of Compliance

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ABclonal:Western blot - PSTPIP1 Rabbit pAb (A7760)}

Western blot - PSTPIP1 Rabbit pAb (A7760)

Western blot analysis of various lysates using PSTPIP1 Rabbit pAb (A7760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.
ABclonal:Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)}

Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)

Immunohistochemistry analysis of paraffin-embedded Human stomach using PSTPIP1 Rabbit pAb (A7760) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)}

Immunohistochemistry - PSTPIP1 Rabbit pAb (A7760)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PSTPIP1 Rabbit pAb (A7760) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)