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PTCH1 Rabbit pAb (A0826)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PTCH1 Rabbit pAb (A0826)

Western blot analysis of lysates from Mouse lung, using PTCH1 Rabbit pAb (A0826) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Human breast using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PTCH1 Rabbit pAb (A0826)

Immunofluorescence analysis of paraffin-embedded mouse embryos using PTCH1 Rabbit pAb (A0826) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePTCH1 Rabbit pAb
Catalog No.A0826
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).
SequenceTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLW
Gene ID
Swiss Prot
SynonymsPTC; BCNS; PTC1; PTCH; NBCCS; PTCH1
Calculated MW161kDa
Observed MW161kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse lung
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PTCH1 Rabbit pAb (A0826)}

Western blot - PTCH1 Rabbit pAb (A0826)

Western blot analysis of lysates from Mouse lung, using PTCH1 Rabbit pAb (A0826) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)}

Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)}

Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PTCH1 Rabbit pAb (A0826)}

Immunohistochemistry - PTCH1 Rabbit pAb (A0826)

Immunohistochemistry analysis of paraffin-embedded Human breast using PTCH1 Rabbit pAb (A0826) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - PTCH1 Rabbit pAb (A0826)}

Immunofluorescence - PTCH1 Rabbit pAb (A0826)

Immunofluorescence analysis of paraffin-embedded mouse embryos using PTCH1 Rabbit pAb (A0826) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (25)