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PTPIP51/RMDN3 Rabbit pAb (A5820)

Review (1)Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PTPIP51/RMDN3 Rabbit pAb (A5820)

Western blot analysis of various lysates using PTPIP51/RMDN3 Rabbit pAb (A5820) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of NIH/3T3 cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of PC-12 cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of U2OS cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for PTPIP51/RMDN3 Rabbit pAb(A5820)

Overview

Product namePTPIP51/RMDN3 Rabbit pAb
Catalog No.A5820
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables microtubule binding activity. Involved in cellular calcium ion homeostasis. Located in several cellular components, including intercellular bridge; mitochondrial outer membrane; and spindle.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 171-470 of human PTPIP51/RMDN3 (NP_060615.1).
SequenceESEGGYTTANAESDNERDSDKESEDGEDEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCHLWYAVLCGQLAEHESIQRRIQSGFSFKEHVDKAIALQPENPMAHFLLGRWCYQVSHLSWLEKKTATALLESPLSATVEDALQSFLKAEELQPGFSKAGRVYISKCYRELGKNSEARWWMKLALELPDVTKEDLAIQKDLEELEVILRD
Gene ID
Swiss Prot
SynonymsRMD3; RMD-3; FAM82C; FAM82A2; ptpip51; PTPIP51/RMDN3
Calculated MW52kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, HeLa, MCF7, Mouse liver, Mouse brain, Mouse kidney
Cellular locationCytoplasm, Mitochondrion membrane, Mitochondrion outer membrane, Nucleus, Single-pass membrane protein, cytoskeleton, spindle, spindle pole.
Customer validation

(Mouse heart,Rat heart)

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Western blot - PTPIP51/RMDN3 Rabbit pAb (A5820)

Western blot analysis of various lysates using PTPIP51/RMDN3 Rabbit pAb (A5820) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunohistochemistry - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of NIH/3T3 cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of PC-12 cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)}

Immunofluorescence - PTPIP51/RMDN3 Rabbit pAb (A5820)

Immunofluorescence analysis of U2OS cells using PTPIP51/RMDN3 Rabbit pAb (A5820) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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