제품 > 항체 > 단일클론항체(mAb)

PUMA Rabbit mAb (A3752)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PUMA Rabbit mAb (A3752) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PUMA Rabbit mAb (A3752)

Immunohistochemistry analysis of paraffin-embedded Rat brain using PUMA Rabbit mAb (A3752) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using PUMA Rabbit mAb (A3752) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PUMA Rabbit mAb (A3752)

Confocal imaging of NIH/3T3 cells using PUMA Rabbit mAb (A3752, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of U-2 OS cells using PUMA Rabbit mAb (A3752, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	PUMA Rabbit mAb
Catalog No.	A3752
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0247

Background

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 100-193 of human PUMA (NP_055232.1)
Sequence	AEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN
Gene ID
Swiss Prot
Synonyms	JFY1; PUMA; JFY-1
Calculated MW	18kDa
Observed MW	18kDa/23kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 IHC-P 1:100 - 1:1000 IF/ICC 1:100 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, A549, K-562, LO2, NIH/3T3, Mouse lung, Mouse spleen, Rat liver
Cellular location	Mitochondrion.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus) IP(mus musculus) IF(Mus musculus) IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

연구세포 생물학 & 발달 생물학세포사멸세포사멸의 미토콘드리아 조절

PUMA Rabbit mAb images

Western blot - PUMA Rabbit mAb (A3752)

Immunohistochemistry - PUMA Rabbit mAb (A3752)

Immunofluorescence - PUMA Rabbit mAb (A3752)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

35847946

Title:

Hymeglusin Enhances the Pro-Apoptotic Effects of Venetoclax in Acute Myeloid Leukemia - PMC

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

35789855

Title:

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism - PMC

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Experimental cell research

Application:

IF:

4.14

Species:

Homo sapiens

PMID:

36442518

Title:

A deuterohemin peptide protects cerebral ischemia-reperfusion injury by preventing oxidative stress in vitro and in vivo

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Autoimmunity

Application:

IF:

2.95

Species:

Homo sapiens

PMID:

36852641

Title:

Endonuclease G promotes preeclampsia by regulating the Wnt signaling pathway

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

American journal of physiology. Renal physiology

Application:

IF:

4.09

Species:

Mus musculus

PMID:

37141146

Title:

YAP promotes AP-1 expression in tubular epithelial cells in the kidney

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Human reproduction (Oxford, England)

Application:

IF:

6.35

Species:

Homo sapiens

PMID:

37451671

Title:

Inhibition of checkpoint kinase prevents human oocyte apoptosis induced by chemotherapy and allows enhanced tumour chemotherapeutic efficacy

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

36116323

Title:

Design and synthesis of N-(1-(6-(substituted phenyl)-pyridazin-3-yl)-piperidine-3-yl)-amine derivatives as JMJD6 inhibitors

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

mus musculus

PMID:

37955806

Title:

DLK-MAPK Signaling Coupled with DNA Damage Promotes Intrinsic Neurotoxicity Associated with Non-Mutated Tau

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Research square

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

36945524

Title:

Modeling Alzheimers disease in primary neurons reveals DNA damage response coupled with MAPK-DLK signaling in wild-type tau-induced neurodegeneration - PMC

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Cellular & molecular biology letters

Application:

IF:

8.7

Species:

Rattus norvegicus

PMID:

37438690

Title:

Hypothermic oxygenated perfusion attenuates DCD liver ischemia-reperfusion injury by activating the JAK2/STAT3/HAX1 pathway to regulate endoplasmic reticulum stress - PMC

References for PUMA Rabbit mAb (A3752)

Product:

PUMA Rabbit mAb

Journal:

Toxicol Appl Pharmacol

Application:

WB, IP

IF:

3.8

Species:

Homo sapiens

PMID:

34798143

Title:

Up-regulation of PUMA caused the activation of p53 phosphorylation and acetylation, enhancing the interaction between PUMA and Bcl-X and mediating arsenic