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PWP1 Rabbit mAb (A21143)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PWP1 Rabbit mAb (A21143)

Western blot analysis of various lysates using PWP1 Rabbit mAb (A21143) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PWP1 Rabbit mAb (A21143)

Western blot analysis of various lysates using PWP1 Rabbit mAb (A21143) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product namePWP1 Rabbit mAb
Catalog No.A21143
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3017
The protein encoded by this gene contains several WD-40 repeats and is found mostly in the nucleus. The expression and localization of this protein are cell cycle dependent. Expression of this gene is upregulated in pancreatic adenocarcinoma. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 460-501 of human PWP1 (NP_008993.1).
SequenceISTVSSVNEAFGRRERLVLGSARNSSISGPFGSRSSDTPMES
Gene ID
Swiss Prot
SynonymsNCLB; IEF-SSP-9502; PWP1
Calculated MW56kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Jurkat, Mouse liver, Rat thymus
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PWP1 Rabbit mAb (A21143)}

    Western blot - PWP1 Rabbit mAb (A21143)

    Western blot analysis of various lysates using PWP1 Rabbit mAb (A21143) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - PWP1 Rabbit mAb (A21143)}

    Western blot - PWP1 Rabbit mAb (A21143)

    Western blot analysis of various lysates using PWP1 Rabbit mAb (A21143) at1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A21143? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)