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PYCR2 Rabbit pAb (A15155)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PYCR2 Rabbit pAb (A15155)

Western blot analysis of various lysates, using PYCR2 Rabbit pAb (A15155) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Rat liver using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of C6 cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of HeLa cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of L929 cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePYCR2 Rabbit pAb
Catalog No.A15155
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2).
SequenceMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD
Gene ID
Swiss Prot
SynonymsHLD10; P5CR2; PYCR2
Calculated MW34kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A-431, THP-1
Cellular locationCytoplasm, Mitochondrion.

Documents

Certificate of Compliance

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ABclonal:Western blot - PYCR2 Rabbit pAb (A15155)}

Western blot - PYCR2 Rabbit pAb (A15155)

Western blot analysis of various lysates, using PYCR2 Rabbit pAb (A15155) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)}

Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)}

Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Rat liver using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PYCR2 Rabbit pAb (A15155)}

Immunohistochemistry - PYCR2 Rabbit pAb (A15155)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PYCR2 Rabbit pAb (A15155) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)}

Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of C6 cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)}

Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of HeLa cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PYCR2 Rabbit pAb (A15155)}

Immunofluorescence - PYCR2 Rabbit pAb (A15155)

Immunofluorescence analysis of L929 cells using PYCR2 Rabbit pAb (A15155) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)