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PYGB Rabbit pAb (A6402)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PYGB Rabbit pAb (A6402)

Western blot analysis of various lysates using PYGB Rabbit pAb (A6402) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of HepG2 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of NIH/3T3 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of PC-12 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of PC-3 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PYGB Rabbit pAb (A6402)

Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg PYGB antibody (A6402). Western blot was performed from the immunoprecipitate using PYGB antibody (A6402) at a dilution of 1:1000.

Overview

Product namePYGB Rabbit pAb
Catalog No.A6402
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
SequenceLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD
Gene ID
Swiss Prot
SynonymsGPBB; PYGB
Calculated MW97kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesBT-474, SW620, HT-29, HepG2, Mouse brain, Mouse heart, Mouse lung, Rat brain, Rat heart
Cellular locationcytoplasm, extracellular exosome, extracellular region.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - PYGB Rabbit pAb (A6402)}

Western blot - PYGB Rabbit pAb (A6402)

Western blot analysis of various lysates using PYGB Rabbit pAb (A6402) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)}

Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of HepG2 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)}

Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of NIH/3T3 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)}

Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of PC-12 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PYGB Rabbit pAb (A6402)}

Immunofluorescence - PYGB Rabbit pAb (A6402)

Immunofluorescence analysis of PC-3 cells using PYGB Rabbit pAb (A6402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PYGB Rabbit pAb (A6402)}

Immunoprecipitation - PYGB Rabbit pAb (A6402)

Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg PYGB antibody (A6402). Western blot was performed from the immunoprecipitate using PYGB antibody (A6402) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)