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Pan-Akt Rabbit mAb (A18675)

Review (1)Publications (20) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Pan-Akt Rabbit mAb (A18675)

Western blot analysis of various lysates using Pan-Akt Rabbit mAb (A18675) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

Confocal imaging of C2C12 cells using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

Confocal imaging of paraffin-embedded Human testis tissue using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

Confocal imaging of C6 cells using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - Pan-Akt Rabbit mAb (A18675)

Immunoprecipitation analysis of 200 μg extracts from MCF7 cells using 3 μg Pan-Akt Rabbit mAb (A18675). Western blot was performed from the immunoprecipitate using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:1000.

Review
ABclonal: review for Pan-Akt Rabbit mAb(A18675)

Overview

Product namePan-Akt Rabbit mAb
Catalog No.A18675
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5005-05
Human AKT serine-threonine protein kinase family includes three members AKT1, AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4, 5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human Pan-Akt (NP_005154.2).
SequenceMSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSG
Gene ID
Swiss Prot
SynonymsAKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA
Calculated MW56kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:12000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:100 - 1:400
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7, SH-SY5Y, NIH/3T3, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular locationciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle.
Customer validation

(14 um cryosections from 7 dpf zebrafish larvae)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Rattus norvegicus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan-Akt Rabbit mAb (A18675)}

    Western blot - Pan-Akt Rabbit mAb (A18675)

    Western blot analysis of various lysates using Pan-Akt Rabbit mAb (A18675) at1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)}

    Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

    Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)}

    Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)}

    Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)}

    Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)}

    Immunohistochemistry - Pan-Akt Rabbit mAb (A18675)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)}

    Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

    Confocal imaging of C2C12 cells using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)}

    Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

    Confocal imaging of paraffin-embedded Human testis tissue using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - Pan-Akt Rabbit mAb (A18675)}

    Immunofluorescence - Pan-Akt Rabbit mAb (A18675)

    Confocal imaging of C6 cells using Pan-Akt Rabbit mAb (A18675, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunoprecipitation - Pan-Akt Rabbit mAb (A18675)}

    Immunoprecipitation - Pan-Akt Rabbit mAb (A18675)

    Immunoprecipitation analysis of 200 μg extracts from MCF7 cells using 3 μg Pan-Akt Rabbit mAb (A18675). Western blot was performed from the immunoprecipitate using Pan-Akt Rabbit mAb (A18675) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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