제품 > 항체 > 다중클론항체(pAb)

Peroxiredoxin 6 Rabbit pAb (A2031)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Peroxiredoxin 6 Rabbit pAb (A2031) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Peroxiredoxin 6 Rabbit pAb (A2031)

Immunofluorescence analysis of HeLa cells using Peroxiredoxin 6 Rabbit pAb (A2031).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product name	Peroxiredoxin 6 Rabbit pAb
Catalog No.	A2031
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human Peroxiredoxin 6 (NP_004896.1).
Sequence	MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP
Gene ID
Swiss Prot
Synonyms	PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m; Peroxiredoxin 6
Calculated MW	25kDa
Observed MW	27kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, HepG2, Jurkat, THP-1, Mouse liver, Mouse kidney, Rat lung, Rat heart
Cellular location	Cytoplasm, Cytoplasmic vesicle, Lysosome.
Customer validation	IHC(Mus musculus) WB(Bos taurus, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Peroxiredoxin 6 Rabbit pAb images

Western blot - Peroxiredoxin 6 Rabbit pAb (A2031)

Immunofluorescence - Peroxiredoxin 6 Rabbit pAb (A2031)

Immunofluorescence analysis of HeLa cells using Peroxiredoxin 6 Rabbit pAb (A2031).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.