Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from C6 cells, using Phospho-ARHGEF2-S886 Rabbit pAb (AP0926) at 1:1000 dilution.C6 were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
| Product name | Phospho-ARHGEF2-S886 Rabbit pAb |
|---|---|
| Catalog No. | AP0926 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S886 of human ARHGEF2 (NP_001155855.1?). |
|---|---|
| Sequence | RRSLP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | GEF; Lfc; P40; GEFH1; LFP40; GEF-H1; NEDMHM; Phospho-ARHGEF2-S886 |
| Calculated MW | 112kDa |
| Observed MW | 120kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | C6 |
| Cellular location | Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, cytoskeleton, ruffle membrane, spindle, tight junction |
* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1709544607 "ABclonal:Western blot - Phospho-ARHGEF2-S886 Rabbit pAb (AP0926)")
