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Phospho-ATM-S1981 Rabbit mAb (AP1030)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Confocal imaging of paraffin-embedded Human colon tissue(untreated) and Human colon tissue(treated with λpp) using Phospho-ATM-S1981 Rabbit mAb (AP1030, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Western blot - Phospho-ATM-S1981 Rabbit mAb (AP1030)

Western blot analysis of lysates from 293T cells, using Phospho-ATM-S1981 Rabbit mAb (AP1030) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product name	Phospho-ATM-S1981 Rabbit mAb
Catalog No.	AP1030
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1575

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S1981 of human ATM (Q13315).
Sequence	EGSQS
Gene ID
Swiss Prot
Synonyms	AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; Phospho-ATM-S1981
Calculated MW	351kDa
Observed MW	350kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T treated by UV
Cellular location	Cytoplasmic vesicle, Nucleus.