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Phospho-C/EBPB-T235 Rabbit pAb (AP1055)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-C/EBPB-T235 Rabbit pAb (AP1055) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-C/EBPB-T235 Rabbit pAb
Catalog No.	AP1055
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T235 of human C/EBPB (NP_005185.2).
Sequence	PGTPS
Gene ID
Swiss Prot
Synonyms	TCF5; IL6DBP; NF-IL6; C/EBP-beta; Phospho-C/EBPB-T235
Calculated MW	36kDa
Observed MW	38kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Sus scrofa, Homo sapiens)