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Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at 1:1000 dilution or CRKL antibody (A11735). K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-CRKL-Y207 Rabbit pAb (AP0824)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-CRKL-Y207 Rabbit pAb (AP0824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-CRKL-Y207 Rabbit pAb
Catalog No.	AP0824
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
Sequence	HAYAQ
Gene ID
Swiss Prot
Synonyms	CRKL; Phospho-CRKL-Y207
Calculated MW	34kDa
Observed MW	38kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	K-562
Cellular location	cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm