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Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-CRMP2/DPYSL2-T514 Rabbit pAb
Catalog No.AP0821
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic phosphorylated peptide around T514 of human CRMP2/DPYSL2 (NP_001377.1).
SequenceTVTPA
Gene ID
Swiss Prot
SynonymsDRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2; Phospho-CRMP2/DPYSL2-T514
Calculated MW62kDa
Observed MW69kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, PC-12
Cellular locationCytoplasm, Membrane, cytoskeleton, cytosol

Documents

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ABclonal:Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)}

Western blot - Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821)

Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (AP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)