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Phospho-DDR1-Y792 Rabbit pAb (AP1072)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-DDR1-Y792 Rabbit pAb (AP1072)

Western blot analysis of various lysates using Phospho-DDR1-Y792 Rabbit pAb (AP1072) at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-DDR1-Y792 Rabbit pAb
Catalog No.AP1072
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenA synthetic phosphorylated peptide around Y792 of human DDR1 (NP_054699.2).
SequenceNLYAG
Gene ID
Swiss Prot
SynonymsCAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; Phospho-DDR1-Y792
Calculated MW101kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by ATP, NIH/3T3 treated by ATP, C6 treated by ATP
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - Phospho-DDR1-Y792 Rabbit pAb (AP1072)}

Western blot - Phospho-DDR1-Y792 Rabbit pAb (AP1072)

Western blot analysis of various lysates using Phospho-DDR1-Y792 Rabbit pAb (AP1072) at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)