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Phospho-FOXO1-S319 Rabbit pAb (AP1090)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-FOXO1-S319 Rabbit pAb (AP1090) at 1:1000 dilution or FOXO1 antibody (A13862). HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-FOXO1-S319 Rabbit pAb (AP1090)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-FOXO1-S319 Rabbit pAb (AP1090) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-FOXO1-S319 Rabbit pAb (AP1090) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-FOXO1-S319 Rabbit pAb (AP1090) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-FOXO1-S319 Rabbit pAb
Catalog No.	AP1090
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S319 of human FOXO1 (NP_002006.2).
Sequence	TSSNA
Gene ID
Swiss Prot
Synonyms	FKH1; FKHR; FOXO1A; Phospho-FOXO1-S319
Calculated MW	70kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa treated by FBS
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens,Mus musculus)