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Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb (AP1530)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb (AP1530) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by λ-PP mixed solution (1μL) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product name	Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb
Catalog No.	AP1530
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC68446

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y216&Y279 of human GSK3β/GSK3α (NP_063937.2).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	GSK3B; GSK 3 beta; GSK3A; GSK 3 alpha; Phospho-GSK3α-S21+GSK3β-S9
Calculated MW	51kDa/46kDa
Observed MW	46kDa/51kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 cells treated by λ-PP
Cellular location	Cell membrane, Cytoplasm, Nucleus.