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Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0512)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293T cells, using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0512) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Review

Overview

Product name	Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb
Catalog No.	AP0512
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y216 of human GSK3β (NP_001139628).
Sequence	VSYIC
Gene ID
Swiss Prot
Synonyms	GSK3B; gsk-3β; Phospho-GSK3β-Y216 + GSK3α-Y279
Calculated MW	47kDa
Observed MW	50kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293 treated by Insulin
Cellular location	Cell membrane, Cytoplasm, Nucleus.
Customer validation	(Panc-1)