제품 > 항체 > 인산화 특이적 항체

Phospho-IGF1R-Y1280 Rabbit pAb (AP0368)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293 cells, using Phospho-IGF1R-Y1280 Rabbit pAb (AP0368).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-IGF1R-Y1280 Rabbit pAb
Catalog No.	AP0368
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding Y1280 of human IGF1R
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	IGFR; CD221; IGFIR; JTK13; Phospho-IGF1R-Y1280
Calculated MW	155kDa
Observed MW	95kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293
Cellular location	Cell membrane, Single-pass type I membrane protein