제품 > 항체 > 인산화 특이적 항체

Phospho-IKKα-S180 Rabbit pAb (AP0506)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells, using Phospho-IKKα-S180 Rabbit pAb (AP0506) at 1:1000 dilution. HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	Phospho-IKKα-S180 Rabbit pAb
Catalog No.	AP0506
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S180 of human IKKα (NP_001269.3).
Sequence	CTSFV
Gene ID
Swiss Prot
Synonyms	BPS2; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; Phospho-IKKα-S180
Calculated MW	85kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by TNF-α and Calyculin A
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus)