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Phospho-IRE1-S724 Rabbit pAb (AP0878)

Publications (22) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IRE1-S724 Rabbit pAb (AP0878)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRE1-S724 Rabbit pAb (AP0878) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-IRE1-S724 Rabbit pAb (AP0878)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-IRE1-S724 Rabbit pAb (AP0878) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Overview

Product namePhospho-IRE1-S724 Rabbit pAb
Catalog No.AP0878
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
ImmunogenA synthetic phosphorylated peptide around S724 of human IRE1alpha (NP_001424.3).
SequenceGRHSFS
Gene ID
Swiss Prot
SynonymsIRE1; IRE1P; IRE1a; hIRE1p; Phospho-IRE1-S724
Calculated MW110kDa
Observed MW126kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3
Cellular locationEndoplasmic reticulum membrane, Single-pass type I membrane protein.
Customer validation

WB(Mus musculus, Rattus norvegicus, Trachemys scripta elegans, Homo sapiens, Bos taurus, Chlorocebus aethiops, Anatinae)

IHC(Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-IRE1-S724 Rabbit pAb (AP0878)}

    Western blot - Phospho-IRE1-S724 Rabbit pAb (AP0878)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRE1-S724 Rabbit pAb (AP0878) at 1:800 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - Phospho-IRE1-S724 Rabbit pAb (AP0878)}

    Immunohistochemistry - Phospho-IRE1-S724 Rabbit pAb (AP0878)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-IRE1-S724 Rabbit pAb (AP0878) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0878? Please let us know so that we can cite the reference in this datasheet.

    항체 (3)

    Secondary Antibodies (26)